The purpose of this course is designed to train graduate students to understand the experimental designs, general methodologies and techniques in related to the isolation, purification and structure elucidation of natural products. Subjects included preparation of materials, extraction, concentration, partition, column chromatography, recrystallization, high performance liquid chromatography, nuclear magnetic resonance spectrometer (NMR), infrared spectrometer (IR), and ultraviolet/visible spectrophotometer (UV-vis).