1. General principles of vaccination 2. Vaccination strategies in aquaculture 3. Important considerations for fish vaccines 4. Influence of environmental parameters on vaccination 5. Types of vaccines in aquaculture 6. Adjuvants and their mechanism of actions 7. Vaccine formulation, vaccine composition, quality control 8. Immunization against bacterial, viral pathogens and parasites 9. Vaccination failure and adverse effects of vaccination 10. Biosafety and regulatory requirements for fish vaccines 11. Future fish vaccines 12. Practical: Preparation of vaccines; quality control; vaccine administration by different routes; assessment of immune response to vaccination- agglutination test, ELISA, challenge studies