The research related to aquatic animals are very diverse and have developed rapidly in the decades. The aquatic animal research models could be applied on research topics such as nutrition, metabolism, diseases, and environmental pollutions etc.. However, because of the issue such as availability of facilities, funding, animal sources, and animal welfare etc., the accessibility for researchers to conduct in vivo research on aquatic animals is limited. Therefore, in vitro research models based on cells have become an unavoidable and high-quality choice for researchers. The content of this cell culture course ranges from the knowledge about cell nutrition, physiology to technical applications as well as practical series from establish and disinfect a culture system, isolate and culture primary cell to the cryopreservation of cell lines. We hope to provide the students with a comprehensive set of knowledge and practical skills about aquatic animal cell culture which could provide you with a set of powerful tools in future research.