The object of this exercise is to acquaint the student with the basic techniques of microorganisms, and recognize the fundamental characteristics of environmental microorganisms and the effects of environmental factors on microbes, and the demonstrate the role of microorganisms in environmental pollution control. This exercise includes: 1. Microscopic examination 2. Isolation and culture of microbes 3. Simple stain 4.Gram’s stain 5.Growth measurement of microbes 6.Temperature on microbial growth 7.Algae 8.Microbes in sludge 9.Qualty analysis of water indicated microorganism 10.Quantity analysis of water indicated microorganisms 11.Environmentl factors on eutriphication 11.Isolation of cellulomonas.