LabVIEW 2020 has incorporated more powerful wireless communication functions and has the ability to remotely monitor. It adds the editing function of Python program, which is a brand-new architecture system. Significant changes in the program are presented in the loop structure, system monitoring, and image processing. Many new functions have been added. The hardware support is also more mature and complete than previous versions. Each unit is carried out in a step-by-step manner. Provide examples in each unit for learners to operate and practice to improve the learning effect. In response to the fact that learners can more clearly distinguish the status of the connection between the program and the data when practicing and editing the program. The instructor uploads the teaching materials digitally and audio-visually to the digital learning platform Moodle 3.5 for students' reference and practice. This course will introduce the structure of two special nodes (Node), namely MathScript Node and Python Node. Different from the use of Expression Node and the function of Formula Node, which are explained in detail in Graphic Control Programming and Practice 1, both of which are simple mathematical operation nodes, and cannot directly read or execute Mathlab code. Only the MathScript Node of this course can directly load the .m code after clicking Import. GPIB is widely used for communication between computers. Its main purpose is to provide programmable and connected control equipment, so that the controller and the equipment can be more easily matched and applied. The fast and standard communication interface developed can connect the instrument with different controllers. Therefore, the IEEE standard digital interface can be used in programmable instruments. The main application areas include interface systems in different fields such as electronics, electrical engineering, and mechanical engineering.