This course is designed to introduce useful techniques and applications of plant cell and tissue culture. The sterile concepts and industrial application techniques are intended to be established in this course. The content includes totipotency and morphogenesis, the principles of micropropagation, genetics and variations, basic techniques of plant tissue culture, common techniques of plant tissue and organ culture, cell culture, protoplast culture and fusion, hybrid cell selection and identification, applications of hybrid cells, principles, techniques, and applications of artificial seeds, principles and techniques of cryopreservation, and techniques and applications of transgenic plants.