Microbiological knowledge is widely used in medicine, agriculture, fisheries, food, and is inseparable from human life. The goal of this course is to inspire students' understanding of the significance of microorganisms as vital biological resources and their contribution to human health. The contents include the introduction of concepts of applied microbiology, the classification, identification and preservation of microorganisms, the growth and metabolism regulation and other applications, and the applied microorganisms in the food industry, agriculture, chemical industry, pharmaceutical industry, and environmental protection industry.