This laboratory course is to introduce basic techniques and concepts on microbiology as a basis for student to study advanced courses such as the clinical microbiology and other applied courses. The contents include Gram’s stain, acid fast stain, capsule stain, spore stain, preparations of medium, aseptic manipulation technique, bacterial inoculation and purification, bacterial growth and quantitation, effect of antimicrobial drugs on bacterial growth, biological tests, diagnostic kits and culture of anaerobic bacteria.