1. Introduction to bioinformatics 2. Application of bioinformatics to various field 3. Integration of biological data 4. Practical application of Bioinformatics in aquaculture research 5. Tools for similarity search and sequence alignment 6. Alignment of multiple sequences 7. Gene identification and prediction 8. Introduction to genome browser 9. Bacterial genome annotation and virulence determination 10. Metagenomics and its application to microbiome