The purpose of this course is to acquaint students with the principles of plant biotechnology and the current status of applying plant biotechnology in various fields as well as possible advancement in the future. The main subjects include gene structure and expression, plant transformation techniques, the application of molecular markers in plant breeding, and the application of plant biotechnology in basic biology research, plant resistance to biotic and abiotic stresses, food industry, environmental protection, and pharmaceutical development. Safety and regulations regarding transgenic plants are also discussed.