The primary objective of this course is for students to (1) learn fundamental approaches for experimentally investigating biochemical problems, (2) learn the theoretical foundations for the methods used, and (3) understand the applicability of the biochemical methods to realistic situations. Topics covered in this course include: 1. Methods for the isolation of biomolecules, such as proteins and nucleic acids; 2. Characterization of amino acids, proteins (including enzymes) and carbohydrates, and 3. Manipulation of macromolecular structures from databases using visualization software and online resources.Students will also learn how to operate the some instruments such as pH meter, spectrophotometer in this course. It includes buffer preparation, titration of amino acid, qualitative and quantitative analysis of amino of amino acid and protein, quantitative analysis of carbohydrates.