The aim of this lab is to acquaint students with the principles, practices, and application of the methodology routinely employed in plant biotechnology. The contents cover plant gene cloning, transformation and expression. The individual topics include plant DNA and RNA extraction, vector construction, bacterium transformation, plasmid isolation, gene sequence analysis and database searching, Agrobacterium culture and transformation, plant transformation, transformant confirmation, and recombinant protein expression and purification.
- Teacher: 陳幼光 Chen Yu-Kuang
- Teacher: 江主惠 Chu-Hui Chiang
- Teacher: 洪國翔 Hung Kuo-Hsiang
- Teacher: 徐志宏 Shyu Jyh-Horng