Taiwan has important location and unique landscape. There are many treasure embedded in ecological and cultural resources. How to preserve and conserve those resources are quite important research topics. Ecotourism is a new concept to deal with environmental conservation, tourism, and community development. It is not only domestic and foreign trend; also provide turning points for various ecological and cultural industries development. Of this curriculum mainly covers five parts, namely introduction, the ecotourism resources and the investigation, recreational impact and the monitoring, the eco-tourism planning and management, as well as the case studies and reconsiderations. It can enhance the students’ research and operation ability on ecotourism.